Fig. 2: The BRCT domains of BRCA1 and BARD1 bind to pre-rRNAs.
From: The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination
a Schematic diagram for identification of the BRCA1/BARD1 complex-associated RNA species. b Pie charts showing the BRCA1/BARD1 complex-associated RNA species. c Interactions between pre-rRNA and the BRCA1/BARD1 complex are validated by PAR-CLIP and RT-qPCR. d, e Interactions between the recombinant BRAC1-BRCT and BARD1 BRCT and pre-rRNA were examined by GST pull-down assays. GST, GST-BRCA1 BRCT, or GST-BARD1 BRCT proteins were incubated with pre-rRNA. Following GST pull-down assays, RT-qPCR was performed to examine the enrichment of pre-rRNA. Values are means ± SD of three independent assays. f The BRCT domains of BRCA1 and BARD1 directly interact with rRNA. Binding affinities between the BRCT domains and biotin-labeled RNA oligos (25nt) were measured by BLI assays.
