Fig. 4: Characterization of peripheral neutrophils at different stages of FM.

a–c UMAP embedding of peripheral blood immune cells colored by manually annotated clusters. UMAP embedding of peripheral neutrophils colored by manually annotated clusters (b) and samples (c). d FeaturePlot of marker genes of cardiac neutrophils. e GO analysis of specifically expressed genes in peripheral neutrophil clusters. f Cell proportion change of peripheral neutrophils at different time points. g Immunofluorescence staining of peripheral neutrophils at different time points. Scale bars: 20 μm. h, i Developmental trajectories of cardiac and peripheral neutrophils at day 7 colored by pseudotime (h) and cell types (i). j Spearman correlation of cardiac and peripheral neutrophil subtypes. k Box plot of characteristic scores of peripheral neutrophils at different time points. l Box plot of characteristic scores between cardiac and peripheral neutrophils.