Fig. 1: Overall architecture of mouse sperm DMT.
From: In-cell structural insight into the stability of sperm microtubule doublet
a Schematic representation of in-cell structural determination of mouse sperm DMT. The transverse (a) and vertical (b) sections of mouse sperm axoneme are shown as the indicated slices in cryo-electron tomograms, respectively. CPC, central pair complex. DMT, microtubule doublet. A high resolution cryo-EM map of DMT with a spatial periodicity of 48 nm was obtained by sub-tomogram analysis. b Molecular dissection of DMT architecture showing with two 48 nm repeats of DMT cryo-EM map. The layers of the DMT structure were sequentially removed from bottom to top to reveal its inner composition. The tubulin subunits as well as MIP subunits are colored and labeled individually. The color schemes are summarized in Supplementary Table S5. To be noted, the subunit CFAP107 is hidden by other MIPs and could not be shown in the current view. c List of names and functions of tubulins and MIPs from three representative specimen, Chlamydomonas reinhardtii, Bovine trachea, and Mus musculus sperm. Different colors represent different functional roles of MIPs in the architecture of DMT.
