Fig. 4: Interactions between MIPs and Tektin bundles in A-tubule and between MIPs in B-tubule.
From: In-cell structural insight into the stability of sperm microtubule doublet
a Interactions with 16 nm periodicity. The vertical sections of DMTF16 map at the A-tubule and B-tubule are shown, respectively, and the MIPs responsible for anchoring Tektin bundles onto the tubulin wall in A-tubule, as well as the MIPs interacting with the ribbons in B-tubule, are highlighted and colored accordingly (Supplementary Table S5). The Tektin bundles and tubulin wall are shown in low light and transparency. The interaction interfaces with residues labeled between Tektin5-4 and EFHC2, Tektin1-1 and EFHC1, Tektin1-1 and FAM166C in A-tubule are zoomed in and shown in Panel I-III, respectively. All contacting residues were recognized using default parameters in UCSF ChimeraX48. b Interactions with 48 nm periodicity. The vertical sections of DMTF48 map at the A-tubule is shown and the MIPs responsible for anchoring Tektin bundle onto the tubulin wall are highlighted and colored accordingly (Supplementary Table S5). The Tektin bundles and tubulin wall are hidden. The interactions between Tektin5-7 and CFAP53, among Tektin5-3 filament, Tektin3-3 filament, MNS1, CFAP107, and CFAP161, among Tektin5-5 filament, Tektin3-3 filament, SPAG8, NME7, and CFAP53, and among Tektin5-5 filament, Tektin3-3 filament, CFAP141, and NME7, are zoomed in and highlighted in Panel I–IV with vertical and transverse section views, respectively. c A schematic model of MIPs interactions in A-tubule.
