Fig. 4: SHIP164 depletion strongly affects Rab14 EE, and RhoBTB3 or Vps26B depletion phenocopies the SHIP164 depletion. | Cell Discovery

Fig. 4: SHIP164 depletion strongly affects Rab14 EE, and RhoBTB3 or Vps26B depletion phenocopies the SHIP164 depletion.

From: Biogenesis of Rab14-positive endosome buds at Golgi–endosome contacts by the RhoBTB3–SHIP164–Vps26B complex

Fig. 4

a Representative images of live HEK293 cells expressing Halo-Rab14 upon scrambled or two independent SHIP164 siRNAs with two insets on the right. b–h Representative images of live HEK293 cells expressing multiple markers for endosomal subpopulations, including Halo-Rab4 (b), Halo-Rab5 (c), Halo-Rab17 (d), Halo-Rab21 (e), Halo-Rab22 (f), Halo-Rab23 (g), or GFP-Rab7 (h), upon scrambled or SHIP164 siRNA with two insets, respectively. i, j The size (i) or number (j) of EEs per cell in scrambled or SHIP164 siRNA-treated cells based on studies in a–h. More than 20 cells from three independent assays were quantified for each condition. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± SD. k Representative images of live HEK293 cells expressing Halo-Rab14 upon two different siRNAs targeting RhoBTB3 (upper) or Vps26B (bottom) with two insets on the right. l, m Immunoblot analyses showing the efficiency of RhoBTB3 (l) or Vps26B (m) depletion via two siRNAs. n The number (left) or size (right) of EEs per cell in scrambled (26 cells), RhoBTB3 (34 cells) or Vps26B (33 cells) siRNA-treated cells. Three independent assays were quantified for each condition. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± SD. Scale bars: 10 μm in the whole cell images and 2 μm in the insets (a–h, k).

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