Fig. 5: ANKFY1 promotes ATG2A-mediated lipid transfer in vitro.
From: ANKFY1 bridges ATG2A-mediated lipid transfer from endosomes to phagophores
a Assessment of liposome size distribution by DLS. The 100 nm liposomes were reconstituted and incubated with the indicated proteins at 37 °C. b Schematic diagram of lipid transfer assay. Donor liposomes (61% DOPC, 30% POPE, 2% NBD-PE, 2% Rhod-PE, 5% PI3P) and acceptor liposomes (65% DOPC, 30% POPE, 5% PI3P) were mixed with ATG2A, ANKFY1 or both as indicated. c FRET-based lipid transfer assay as in b. The lipid transfer was measured by the increase of NBD fluorescence intensity. d Quantification of results in c. Bars represent averages of the NBD signal observed after 2000 s in three independent experiments. Error bars represent standard deviations. Data are mean ± SD. **P ≤ 0.01; ****P ≤ 0.0001; ns, not significant.
