Fig. 8: Identification of REST as a potential marker for NE-low SCLC associated with immune infiltration and patient survival. | Cell Discovery

Fig. 8: Identification of REST as a potential marker for NE-low SCLC associated with immune infiltration and patient survival.

From: Single-cell and spatial proteo-transcriptomic profiling reveals immune infiltration heterogeneity associated with neuroendocrine features in small cell lung cancer

Fig. 8

a The consensus map of the patients based on various statistical metrics decomposed by NMF. b GSVA enrichment fraction of the Cancer Hallmark processes in each patient, with 3 NMF-clustered groups represented (top). Relationship between the 3 NMF groups and immune cell infiltration in tumor stroma and tumor nests, respectively (middle two). Relationship between the 3 NMF groups and ASCL1, POU2F3, NEUROD1, and REST expression (Bottom). c Relationship between the four NMF subtypes and immune cell infiltration at ROI level. d Enrichment of transcription factors of potential regulatory genes that are differentially expressed, and clustered by patients. e Enrichment of transcription factors of potential regulatory genes that are differentially expressed, and clustered by ROIs. f Kaplan–Meier survival curves of LS-SCLC patients stratified by the REST expression level (at the optimal cutoff point) in the current DSP cohort and an external independent validation cohort of 42 patients. g Spearman correlation of the expression of previously defined molecular subtyping marker genes ASCL1, POU2F3, NEUROD1, and REST with infiltration of major immune subsets in all tumor nest ROIs.

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