Fig. 5: SNRNP200 enhances RNA splicing in genes encoding metabolic enzymes with weak 5’ splice sites.

a Enrichment networks of intron-retained transcripts in control and SNRNP200-knockdown MDA-MB-231 cells were analyzed and visualized by Metascape. Representative terms with kappa similarities above 0.3 formed a network and were depicted using Cytoscape software. b Sashimi plots depicting the RIs of GAPDH (top) and GSS (bottom). The number of RI reads is indicated (control sgRNA in blue, SNRNP200 sgRNA in red). c Representative RT-PCR validation of SNRNP200-regulated RI events in MDA-MB-231 cells. The structure of each isoform is illustrated in the diagrams. Individual data points are presented (n = 3; *P < 0.05; *P < 0.01; **P < 0.001; Student’s t-test). d A 5’ splice site strength analysis of RI events was carried out. Non-retained introns (NRIs) within the same set of genes were used for comparison (Wilcoxon rank-sum test). e Motif enrichment analysis of the same set of genes revealed that the most frequently identified motifs aligned with the consensus 5’ splice site sequences for both the RI and NRI. f The GC compositions of the RI and NRI were calculated by dividing the GC content of each intron by the average of its adjacent exons and compared by the Wilcoxon rank-sum test.