Fig. 4: Myosin-5a mediates transport of migrasome intraluminal vesicles.
From: Localized, highly efficient secretion of signaling proteins by migrasomes
a TEM images of WT, Myo5a OE and Myosin-5a knockout (Myo5a KO) L929 cells. Scale bar, 500 nm. The right panel shows statistical analysis of the number of small vesicles per migrasome. Data are means ± SEM for > 100 migrasomes from three independent experiments. Two-tailed unpaired t-test was used for statistical analyses. ***P < 0.001. b Immunostaining of endogenous Rab11 in WT, Myo5a OE and Myo5a KO L929-GFP-Rab8a cells. Scale bar, 20 μm. Lower panels, enlarged ROI. Scale bar, 2 μm. Right panels, statistical analysis of the number of GFP-Rab8a and Rab11 puncta in migrasomes per cell. Data are means ± SEM. n > 100 cells from three independent experiments. Two-tailed unpaired t-test was used for statistical analyses. ***P < 0.001. c Immunostaining of endogenous VAMP2 in WT, Myo5a OE and Myo5a KO L929 cells. Scale bar, 20 µm. Lower panels, enlarged ROI. Scale bar, 2 μm. Right panel, statistical analysis of the number of VAMP2 puncta in migrasomes per cell. Data are means ± SEM. n > 100 cells from three independent experiments. Two-tailed unpaired t-test was used for statistical analyses. ***P < 0.001. d Stable expression of T4-mCherry or mCherry-Myo5a was established in L929-GFP-VAMP7 cells. The cells were then subjected to confocal analysis. Scale bar, 20 µm. Right panels, enlarged ROI. Scale bar, 2 µm. Statistical analysis of the number of GFP-VAMP7 puncta in migrasomes per cell is shown as the means ± SEM. n > 100 cells from three independent experiments were analyzed using the two-tailed unpaired t-test (right panel). ***P < 0.001.
