Fig. 6: Migrasomes are enriched with cytokines.
From: Localized, highly efficient secretion of signaling proteins by migrasomes
a, b L929-GFP-VAMP2 cells were stained with M-CSF (a) or CCL2 (b) antibody and then visualized. Scale bar, 20 μm. The right panels show enlarged migrasomes. Scale bar, 2 μm. c, d Immunostaining of endogenous M-CSF (c) or CCL2 (d) in WT, Myo5a OE and Myo5a KO L929 cells. Scale bar, 20 µm. Right panels, enlarged ROI. Scale bar, 2 µm. Statistical analysis of the number of M-CSF (c) and CCL2 (d) puncta in migrasomes per cell is shown as the means ± SEM. n > 100 cells from three independent experiments were analyzed using the two-tailed unpaired t-test (right panel). **P < 0.01, ***P < 0.001. e Migrasomes were purified from the indicated L929 cells. Cell lysates and migrasomes were normalized with total protein and subjected to western blot analysis using the indicated antibodies. PIGK was used as a migrasome marker in L929 cells. Representative densitometry analysis of western blot gray values is shown. Three independent experiments were conducted.
