Fig. 3: Intra- and inter-tetranucleosomal interactions in the H5-chromatin fiber.
From: Structural basis for linker histone H5–nucleosome binding and chromatin fiber compaction
a Two tetranucleosomal units of the H5-chromatin are displayed to exhibit the interactions within and between tetranucleosomal units. The regions enclosed by the blue, cyan, and orange dashed lines are illustrated in detail in b, c and e, respectively. b The structural details of nucleosome–nucleosome interactions within tetranucleosomal unit engaged by adjacent H2A–H2B and H2B–H2B pairs, in which H2A (yellow) and H2B (red) are shown in ribbon representation. The key residues involved in the interactions are labeled and shown in sticks. See also Supplementary information, Fig. S8a and Table S1. c The structural details of the nucleosome–nucleosome interactions between tetranucleosomal units engaged by H4 N-tails (green) and the adjacent H2A-H2B acidic patches (red dots). The key residues involved in the interactions on H4 N-tails are numbered and highlighted by blue spheres. d Left: the structural alignments of the H4 N-tails participating in the interactions between tetranucleosomal units. The hinge residue, R23, is highlighted. Right: a “zipper” model for the interactions between H4 N-tails and the adjacent acidic patches. e The structural details of the H5–H5 interaction between tetranucleosomal units. The meshed dots show the interaction regions. See also Supplementary information, Fig. S8b.
