Fig. 2: Rat tracheal epithelial cells can be successfully transduced with VSV-G pseudotyped LV vector. | Gene Therapy

Fig. 2: Rat tracheal epithelial cells can be successfully transduced with VSV-G pseudotyped LV vector.

From: Lentiviral vector gene therapy and CFTR modulators show comparable effectiveness in cystic fibrosis rat airway models

Fig. 2

A Wild-type rat airway cells transduced with LV-GFP at MOIs of 1, 10 and 100 show dose-related increases in the proportion of cells with green fluorescence. B Corresponding flow cytometry quantification of the percentage of GFP-positive cells. C Graph showing the average percentage of GFP-positive cells at the respective MOIs. D Immunostaining with cytokeratin 5 confirmed the identity of the rat airway epithelial cells as predominantly basal. Data are represented as mean ± SEM, one-way ANOVA, Tukey’s multiple comparison test, ****p < 0.0001, n = 6 technical replicates per group (performed over two independent experiments).

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