Fig. 1: Metabolic activity and IL-1Ra production in monolayer-cultured, PCRX-201-transduced, degenerate human NP cells. | Gene Therapy

Fig. 1: Metabolic activity and IL-1Ra production in monolayer-cultured, PCRX-201-transduced, degenerate human NP cells.

From: PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration

Fig. 1

a Metabolic activity of human NP cells 48 h after transduction with PCRX-201 at different multiplicities of infection (MOI), normalised to untreated controls. IL-1Ra production in cell culture supernatant after (b) 2 d PCRX-201 transduction ±1 d 10 ng/mL IL-1β stimulation, c 5 d PCRX-201 transduction ±4 d 10 ng/mL IL-1β stimulation. Horizontal lines represent median values. Significant differences determined by Kruskal–Wallis with Dunn’s multiple comparison tests *p ≤ 0.05.

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