Fig. 3: Viability and downstream protein production in 3D cultured human NP cells following PCRX-201 transduction. | Gene Therapy

Fig. 3: Viability and downstream protein production in 3D cultured human NP cells following PCRX-201 transduction.

From: PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration

Fig. 3

Degenerate human NP cells (n = 6 patients in triplicate) were transduced with PCRX-201 at a multiplicity of infection (MOI) of 0, 750 or 3000, resuspended in alginate beads and cultured for 3 weeks, +/−100 pg/mL IL-1β for the last week of culture. Following culture (a) total DNA/alginate bead was measured to determine effects of culture on NP cell viability. ELISA was used to determine the downstream production of (b) IL-1Ra, (c) IL-1β, (d) IL-6, (e) IL-8, (f) MMP3, (g) ADAMTS4, and (h) VEGF within cell culture supernatant after 3w culture. Horizontal lines represent median values. Significant differences determined by Kruskal–Wallis with Dunn’s multiple comparison tests *p ≤ 0.05.

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