Fig. 4: Paracrine effect on downstream protein production in 3D alginate bead-cultured human NP cells when exposed to PCRX-201-conditioned media. | Gene Therapy

Fig. 4: Paracrine effect on downstream protein production in 3D alginate bead-cultured human NP cells when exposed to PCRX-201-conditioned media.

From: PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration

Fig. 4

Degenerate human NP cells (n = 6 patients in triplicate) encapsulated in alginate beads were treated for 3 days with conditioned media from NP cells transduced with PCRX-201 (MOI:3000) for 1 week previously. Cells treated with media from previously non-transduced cells served as controls (CTR). ELISA was used to determine the effects on downstream production of (a) IL-1β, (b) IL-6, (c) MMP3, (d) ADAMTS4 and (e) VEGF within cell culture supernatant after 3 days of culture. Horizontal lines represent median values. Significant differences determined by Mann-Whitney tests *p ≤ 0.05.

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