Fig. 2: Effect of the Stim1 mutation on SOCE and NFAT activation.
From: Natural genetic variation in Stim1 creates stroke in the spontaneously hypertensive rat
a Average time course for [Ca2+]i influx in response to store-depletion by thapsigargin (TG, 2 μmol/L) followed by Ca2+ re-addition to induce SOCE in peripheral blood mononuclear cells (PBMC). b Summary graph of sustained phases of TG-induced Ca2+ influx. c Confocal microscopy of NFATc1 nuclear translocation in SHR-A3, SHR-B2 and SHR-A3(Stim1-B2) T cells stimulated for 60 min with PMA (10 nmol/L) and Ionomycin (2 μmol/L). Green indicates NFATc1 staining and red indicates nuclear stain using Draq5. d Quantification of IL-2 production by CD3+ T cells from SHR-A3, SHR-B2, and SHR-A3(Stim1-B2) rats in response to PMA (10 nmol/L) and Ionomycin (2 μmol/L) for 4 h. Asterisk (*) indicates P < 0.05 vs SHR-A3 and hash (#) indicates P < 0.05 vs SHR-B2 with n = 5 per group.
