Fig. 3
Expression of Abcc1 in mouse inner ear. (a) Reverse transcription polymerase chain reaction (RT-PCR) analysis of Abcc1 expression in mouse cochlea. RT products without reverse transcriptase were used as negative controls. (b) Expression of Abcc1 was detected in mouse kidney, lung, and cochlea at P3–4. Equal protein loading was confirmed by blotting for α-tubulin. (c) Frozen sections of P28 mouse cochlea show Abcc1 (red) distributed in stria vascularis (SV). Sections were counterstained with DAPI (blue) to identify nuclei. (d) Abcc1 mainly expressed in marginal and intermediate cells of SV. (e) Expression of Abcc1 was detected in mouse auditory nerves. (f, g) Fluorescence was not found in hair cells and spiral ganglion. BM basilar membrane, IHC inner hair cells, OC organ of Corti, OHC outer hair cells, OL osseous labyrinth, SG spiral ganglion, SL spiral ligament, SM scala media, SP spiral prominence, VM vestibular membrane.
