Fig. 3: Screening of ADF genes in pollen that respond to hyperoside.

A Pollen tube germination in the control and treatment with buffer and hyperoside (0.1 mM) groups. The length of the pollen tube (B) and germination rate (C) when treated with buffer and hyperoside for 2 h or not treated. Each treatment had three biological replicates, and error bars display the standard error of the sample. *P < 0.05, **P < 0.01 (Student’s t test). D The gene expression of AeADF1–6 after treatment with buffer and hyperoside (0.1 mM) for 2 h or after no treatment