Fig. 1
From: Activated protein C protects from GvHD via PAR2/PAR3 signalling in regulatory T-cells
aPC ameliorates murine GvHD. a, b Recipient C57BL/6 wild-type (B6) mice or C57BL/6 mice with endogenous high levels of aPC (APChigh) were lethally irradiated (13 Gy) and transplanted with 5 × 106 whole-bone marrow (BM) and 2 × 106 T-cells from donor BALB/c mice (B/cT + BM). Recipient mice were monitored for survival (a Kaplan–Meier curve) and physical parameters (including weight loss, mobility, hunched posture, ruffled fur, and skin integrity), yielding a composite clinical score b; pooled data from three independent experiments each with four recipient mice per genotype. c, d Photomicrographs (c) depicting typical morphology in liver (note lymphocyte infiltration, arrow), small intestine (SI, note apoptotic bodies, arrow head), colon, and skin (note for example loss of hair follicles and epidermic atrophy) and bar graph (d) summarizing histological disease scores. Mean value ± SEM of six B6 recipient and six APChigh-recipient mice d; haematoxylin and eosin stained sections (c size bar: 50 µm); *P < 0.05, **P < 0.01, ***P < 0.001 (a log-rank test; b ANOVA; d t-test)
