Fig. 4

Combining HSP90 inhibition and anti-CTLA4 enhances CD8 T-cell function. a Treatment schedule: ganetespib was administered at 100 mg/kg per mouse once a week and anti-CTLA4 at 100 μg per mouse every 3 days. Two doses of ganetespib and 4 doses of anti-CTLA4 were administered. At day 18, mice were sacrificed, spleens harvested and processed, tumors were harvested and processed for RNA and immune cell infiltration profiled via flow cytometry. b Absolute number of CD8 T cells infiltrating the tumor normalized to tumor weight. c Absolute number of T regulatory cells infiltrating tumor normalized to tumor weight. d Ratio of CD8 T cells to Tregs infiltrating the tumor. e Absolute number of granzyme A positive CD8 T cells normalized to tumor weight. f Absolute number of granzyme B positive CD8 T cells normalized to tumor weight. n = 9 mice. g ELISPOTS showing gp100 and P15E-specific cells from splenocytes of treated mice. The data represented as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by one way ANOVA for B-F or paired two tailed Student’s t-test for G. Data are a representation of at least two independent studies