Fig. 6
From: Endocytic sorting motif interactions involved in Nef-mediated downmodulation of CD4 and CD3
Nef-mediated modulation of cell surface receptors in PBMCs. Flow cytometric analysis of a CD3, b CD69, c CD4, d MHCI, e CXCR4, f CD74 and g CD28 surface expression in PBMCs infected with VSV-G pseudotyped HIV-1 M NL4-3 IRES eGFP recombinants expressing the indicated nef alleles. The eGFP expressing (i.e., infected) population was used to calculate receptor downmodulation. Values were normalized to the nef-deficient control (100%). The mean of three independent infections (three donors) ±SEM is shown in the left column. Examples for primary data with the gating strategy and the MFI of the stained surface receptor are shown on the right
