Fig. 5

Inhibition of the PLCγ pathway impairs IL-7-mediated metabolism of B cell progenitors. a, b BM⁻derived Rag1-deficient B cell progenitors were cultured with IL-7 plus DMSO, CP690550, U73122, or edelfosine. c, d B220⁺IgM⁻IL-7R⁺ B cell progenitors were sorted from wild-type mice and cultured without IL-7 (medium) or with IL-7 plus DMSO, CP690550, U73122, or edelfosine. After measuring basal OCR (a, c) and ECAR (b, d), oligomycin A (A), FCCP (B), and antimycin (C) were sequentially added. Error bars show ± SEM. Data shown are obtained from 5 (a, b) and 3 (c, d) independent experimentsED: On your figures please ensure all instances of PLCy refer to proteins, and <Emphasis Type="Italic">Plcg</Emphasis> is used to refer to the gene.