Fig. 7 | Nature Communications

Fig. 7

From: Oxidation of Atg3 and Atg7 mediates inhibition of autophagy

Fig. 7

Loss in LC3 lipidation and increased Atg7 oxidation in aged mouse aorta. a Aged mice have impaired aortic LC3 lipidation, higher expression of p62, and loss of LC3-Atg7 in response to 24-h food withdrawal. In addition, aged mice had increased Atg7 oxidation in their aortic vessels, at a molecular weight consistent with a disulfide-bound Atg3 heterodimer. The graphs to the right show quantification of LC3 lipidation, p62, Atg7 oxidation, Atg3-LC3, and Atg7-LC3 formation in aorta from fed or starved young or aged mice. b The hyperoxidation of prx (Prx-SO3) was significantly enhanced in aortic vessels of aged mice after 24-h food withdrawal. The graph to the right shows quantification of Prx-SO3 in aorta from fed or starved young or aged mice. c Plasma protein carbonylation is significantly enhanced in aged starved mice. The graph to the right shows quantification of carbonylated plasma proteins from fed or starved young or aged mice. All data represent mean ± s.e. from 8–9 (a, b) mice/group except for analysis of p62 expression and plasma protein carbonylation (c) which was using five mice/group. *P < 0.05, **P < 0.01, statistical significance (Dunnett’s test) relative to young fed mice. †P < 0.05, †††P < 0.005 statistical significance relative to young starved mice

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