Fig. 5
ANGPTL8 facilitates the degradation of IKKγ. a Effects of ANGPTL8 overexpression on the expression level of transfected TRAF6, RIP1, IKKα, IKKβ or IKKγ in HEK293T cells. b Effects of ANGPTL8-RNAi on the expression level of transfected TRAF6, RIP1, IKKα, IKKβ, or IKKγ. c Effects of ANGPTL8-RNAi on the expression levels of the endogenous IKKα, IKKβ or IKKγ after TNFα and CHX co-treatment. The indicated cells were treated by CHX (200 μg/mL) for 1 h, followed by TNFα treatment for the indicated time. d Knockdown of IKKγ inhibits ANGPTL8-RNAi facilitated NF-κB activation triggered by TNFα (2 h) in the ANGPTL8-RNAi or control cell lines (n = 3). e Knockdown of IKKγ inhibits ANGPTL8 deficiency-facilitated NF-κB activation triggered by TNFα for 2 h (n = 3). Data are shown as the mean ± SEM, unpaired two-tailed student’s test was used for statistics (d, e). ***p < 0.001, NS > 0.05. Data are representative of three independent experiments
