Fig. 4
From: Biomimetic temporal self-assembly via fuel-driven controlled supramolecular polymerization
ATP-selective seeded supramolecular polymerization of 1. a CD signal showing seeded supramolecular polymerization of 1 upon two subsequent addition of monomers with ATP (c = 2 × 10−5 M). b Overlaid spectra of CD changes showing the simultaneous (blue) and consecutive (red) addition of 1 and ATP to pre-grown seed. c CD signals showing no changes on addition of 5 equiv. of AMP or 2 equiv. of ADP along with 1 to a pre-grown seed, but increase in CD signal as soon as 0.9 equiv. of ATP was added to it. (Seed conc. [S] = 2 × 10−5 M, fresh feed conc. [1] = 1 × 10−5 M (5 μL of 1 (5 × 10−3 M stock) and 0.45 μL of ATP (10−2 M)). d CD signal showing seeded polymerization for [1]/[S] ratios of 0.33:1, 0.5:1, 1:1, 2:1, and 5.7:1. e Log–log plot of the rate of change in absorbance as a function of [1] (x = 2, 1, 0.5, and 0.33) exhibits a linear relationship with a correlation coefficient of 0.98. Error bars have been shown in red for data achieved from experiments carried out for a maximum of three times. f DLS data showing the increase in hydrodynamic radii from seed (S) to varying [1]/[S] ratios (inset shows the straight line fit of size increase for various seed concentrations where green ball is the seed size and blue balls are the sizes of seeded stacks) ([S] = 1 × 10−5 M, [1] = 0.33 × 10−5 M (1.65 μL), 0.5 × 10−5 M (2.5 μL), 1 × 10−5 M (5 μL), 2 × 10−5 M (10 μL), 5.7 × 10−5 M (2.85 μL) (HEPES/CH3CN, 90/10, v/v, 0.9 equiv. ATP, 30 °C)
