Fig. 8
COP1 and OTUD7B coordinate to control NPCs differentiation. a, b NPCs were plated in Matrigel-coated six-well plate for neuronal differentiation. For the indicated times, immunoblotting (a) and immunofluorescence (b) of Sox2, CUL4A, COP1, OTUD7B and TUJ1 were performed. c Cells transfected with HA-Ub and indicated shRNA were treated by MG132 for 8 h, and the lysates were immunoprecipated with anti-Sox2 antibody and immunoblotted with anti-HA. d Summary of our findings. OTUD7B stabilizes Sox2 through deubiquitylation and maintains the stemness of NPCs, while CUL4ADET1-COP1 promotes Sox2 degradation through ubiquitylation and induces differentiation of NPCs. OTUD7B and CUL4ADET1-COP1 exert opposite roles in regulating Sox2 protein stability at the post-translational level. The representative images are shown from three independent experiments. Unprocessed original scans of blots are shown in Supplementary Fig. 9
