Fig. 9 | Nature Communications

Fig. 9

From: Runx/Cbfβ complexes protect group 2 innate lymphoid cells from exhausted-like hyporesponsiveness during allergic airway inflammation

Fig. 9

Exhausted-like hyporesponsiveness of ILC2s attenuates allergic inflammation. a PCR analysis of the floxed intron 4 in the Cbfb gene compared to the unfloxed genes (control) in Cbfbf/f ERT2-Cre ILC2s cultured with IL-2, IL-7, and IL-33 with or without 4-hydroxytamoxifen (4OHT). b Flow cytometry analysis of IL-5, IL-13, IL-10, and TIGIT expression by Cbfbf/f or Cbfbf/f ERT2-Cre ILC2s cultured with 4OHT. c–f Rag2–/– Il2rg–/– mice were adoptively transferred with Cbfbf/f or Cbfbf/f ERT2-Cre ILC2s (1 × 106 cells/mouse) prepared as in b and administered papain intranasally on days 0, 1, and 2. On day 3, the absolute numbers of eosinophils and ILC2s in the bronchoalveolar lavage (BAL) fluid and lung (c) and the IL-5 concentration in the BAL fluid (d) from the recipient mice were analyzed by flow cytometry and ELISA, respectively. Histology (e) and the frequency (f) of PAS-positive lung epithelial cells in the indicated recipients. In c, d, f, *p < 0.05 and **p < 0.01 by Student’s t-test. Data are representative of at least two independent experiments (mean ± s.d. of four mice in c, d, f)

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