Fig. 6
From: Molecular interactions between Hel2 and RNA supporting ribosome-associated quality control
Assessment of ribosome and polysome association of different Hel2 mutants. a Representative A260 profiles (each curve normalized to a sum of 1) of sucrose density gradients from wild-type and mutant strains (see b for colour code and Supplementary Fig. 9 for triplicate quantification of signal in different fractions. b Immunoblot analysis of tagged Hel2 content in pooled and precipitated sucrose fractions containing different sub-populations of ribosomal components, monosomes or polysomes. Membranes were probed for the HTP-tag (using peroxidase-anti-peroxidase) and for Pgk1 as a control. c Quantification of tagged Hel2 content in each pooled fraction. Data were obtained from three independent biological replicates per strain (independently isolated clones). *p < 0.05, according to Student’s t test (two-tailed, paired, w.r.t. wild-type). See b for colour code
