Fig. 1
From: Chaperone activation and client binding of a 2-cysteine peroxiredoxin
Cryo-EM structure of the mTXNPxred: luciferase complex. a 2D reference-free class averages of heat-treated mTXNPxred in the presence (left) or absence (right) of unfolded luciferase showing additional central density attributed to luciferase in top and side (arrow) views. Scale bar equals 5 nm. Cryo-EM 3D map of the mTXNPxred:luciferase complex (b) and mTXNPx alone (c) resolved to 3.7 and 2.9 Å resolution, respectively shown at σ = 3.3. Top and side views are shown. The L. braziliensis mTXNPx crystal structure (4KB3) was docked into the EM density with additional N-terminal residues, Q31-T35, modeled for mTXNPxred:luciferase. d The unsharpened map of the mTXNPxred:luciferase complex displayed at threshold σ = 1.7 showing central density corresponding to bound client. e N-terminal residues Q31-T35 shown docked into the sharpened mTXNPxred:luciferase map with approximate ___location of client (dashed line) adjacent Q31, M32, Y33, and R34
