Fig. 5
From: Chaperone activation and client binding of a 2-cysteine peroxiredoxin
In vitro cross-linking of mTXNPxred-luciferase complexes. a Intra-protein (purple lines) and inter-protein (cyan lines) cross-link maps using xiNET (Supplementary Data 1). The mTXNPx sequence including the mitochondrial targeting sequence was used for the map. b Mapping of the in vitro cross-linking sites between mTXNPxred and luciferase (shown in orange) and the in vivo cross-linking sites between mTXNPx-Bpa variants and cellular client proteins (red: Group 1 cross-links; pink: Group 2 cross-links, Fig. 4a) onto the quaternary structure of L. infantum TXNPxred. For simplicity, only one of the five dimers that are present in the decamer (subunit A in pink and subunit B in blue) as well as one adjacent subunit on each side (grey) are shown
