Fig. 6
From: Chaperone activation and client binding of a 2-cysteine peroxiredoxin
mTXNPx decamers undergo heat-shock-induced rearrangements. a mTXNPxred (2 µM) either alone or in complex with luciferase (0.2 µM) was incubated in the presence (+) or absence (−) of 40 mM glutaraldehyde at 30 °C or 42 °C for 10 min. The oligomeric status of mTXNPxred was assessed by running the samples on non-reducing SDS-PAGE followed by silver staining. Dimeric mTXNPxox was used as control. In the absence of cross-linker, mTXNPxred migrates in the monomeric form. b Three independent experiments were quantified using ImageJ software. Values represent means ± SD. c mTXNPxred (10 µM) was incubated either alone or in the presence of luciferase (1 µM) for 10 min at 42 °C. One aliquot was immediately applied onto an EM grid while the other aliquot was cooled down before applying onto the grids. d Quantification of the number of decamers present in 10 micrographs for each condition +/− std is shown
