Fig. 4
From: Precise tuning of gene expression levels in mammalian cells
Antigen expression levels determine the anti-tumour immune response in vivo. a Experimental design for in vivo OVA-miSFIT B16-F10 tumour growth experiments. b Analysis of tumour volume over time for four B16-F10 cell lines (3 OVA-miSFIT variant lines and one B16-F10 parental control line) challenged with OT-I CD8+ T-cells (x-axis = number of days from tumour cells injection; mean +/− s.e.m.) c Survival curves for mice injected with B16-F10 lines following the same experimental setup as in a. d Frequency of CD8+, OT-I TILs per tumour (mean +/− s.d., Mann–Whitney U-test, *P < 0.05, **P < 0.01). For experimental setup see Supplementary Figure 8. Source data are provided as a Source Data file
