Fig. 4

STAT5B^N642H transforms γδ T-cells, giving rise to aggressive γδ T-cell leukemia/lymphoma. a Schematic of isolation and transplantation of γδ T-cells from wild-type (WT) or hSTAT5B^N642H transgenic (Tg) mice into C57BL/6NRj recipient mice (WT n = 4, N642H n = 6) by tail vein injection. Two out of six N642H γδ T-cell transplant mice showed signs of disease burden at 3 months post transplantation, and they were sacrificed and analyzed along with one healthy WT γδ T-cell transplant mouse. b Macroscopic comparison of lymph node (LN) and spleen size from recipient mice transplanted with γδ T-cells from hSTAT5B^N642H (n = 2) or WT (n = 1) mice. Axillary, brachial, and inguinal LN and spleen weights were quantified and graphed as mean ± SEM, **p < 0.01 by unpaired two-tailed Student’s t-test (LN), or mean ± range (spleen). c Flow cytometric analysis of the percentage of γδ T-cells in the LNs of recipient mice transplanted with γδ T-cells from hSTAT5B^N642H (n = 2) or WT (n = 1) mice. d Histological analysis using CD3, Ki67, and H&E staining of the liver of recipient mice transplanted with γδ T-cells from hSTAT5B^N642H (n = 2) or WT (n = 1) mice. Original magnification: ×20 and ×40 (insets), scale bars = 100 μm. Source data are provided as a Source Data file