Fig. 5
From: Structural and functional consequences of the STAT5BN642H driver mutation
Structural architecture of human STAT5B and STAT5BN642H. a Crystal structure of the STAT5B unphosphorylated dimer illustrating the CCD (green), DBD (purple), linker ___domain (blue), and SH2 ___domain (red). The top monomer shows residue changes between human STAT5A and human STAT5B in yellow spheres, whereas differences between human STAT5A and murine STAT5A are shown in cyan spheres. Secondary structural elements are appropriately labeled on the bottom monomer. Nomenclature for structural elements in the CCD, DBD, and linker ___domain is established on precedents for STAT5A. b Overlay of human STAT5B with murine STAT5A (gray). c Crystal structure of the STAT5BN642H unphosphorylated dimer with a similar color scheme as in a. The mutated N642H position is indicated by cyan spheres. d The SH2 ___domain of wild-type STAT5B with secondary structural elements labeled based on nomenclature for STAT-type SH2 domains. Different conformations of the STAT5B SH2 ___domain are shown for the wild-type (e) and mutant (f, g). The hydrogen bonding network for the β-sheet backbone in the SH2 ___domain for STAT5B (h) and STAT5BN642H (i, j) are shown in two distinct conformations. Source data are provided as in the PDB: 6MBW (STAT5B) and 6MBZ (STAT5BN642H)
