Fig. 3

Wdr62 deletion in mice results in smaller brain size and reduced NPCs. a Schematic diagram of strategy for Wdr62 null mouse generation. b Western blot analyses of Wdr62 protein expression in mouse embryonic fibroblasts (MEFs) isolated from E14.5 embryos. β-actin serves as the control. c, e Dorsal views of E18.5 and P30 mouse brains. Scale bars: 1 and 2 mm. d Statistical analysis of E18.5 WT (n = 17) or mutant (n = 17) brain weights. *P < 0.05 (Student’s t-test). f Coronal sections of P30 cerebral cortex stained with haematoxylin and eosin. The lower panels are the enlargements of upper panels . g Quantification of radial thickness in neocortex. Error bars indicate SEM of 9 sections at comparable positions along the rostral/caudal axis of cerebral cortex from three independent experiments. *P < 0.05 (Student’s t-test). h, j Confocal microscope images of coronal sections of E16.5 cerebral cortex stained with antibodies against Pax6 (green, h) or Tbr2 (red, j). Hoechst stains nuclei (blue). Lower panels are the enlargements from the white boxed areas in the upper panels. Scale bars: 50 μm. i, k Quantification of the percentage of Pax6-positive and Tbr2-positive cells per total number of NPCs within VZ/SVZ. Error bars represent SEM of 9 sections from three independent experiments. *P < 0.05 (Student’s t-test)