Fig. 7
WDR62 recruits CEP170 to the basal body for promoting cilium disassembly. a Cep170 interacts with Wdr62 by co-IP assay. Bottom panels: schematic diagrams of full length, N-terminal, and C-terminal Wdr62 constructs. b MEFs stained with antibodies against acetylated α-tubulin (green), Cep170 (red), and Wdr62 (purple). Scale bars: 2 μm. c 2D human NPCs stained with antibodies against Arl13b (red) and CEP170 (green). Scale bars: 2.5 μm. d Quantification of CEP170 signal intensity in control (n = 68) and mutant (n = 70) human NPCs with cilia. e Quantification of cilium length of control (n = 51) and Cep170 knockdown (n = 52) MEFs under serum starvation condition. f Quantification of cilium length of MEFs, including WT (n = 80), Wdr62−/− (n = 67), Cep170 knockdown (n = 80), and Cep170 knockdown in Wdr62−/− (n = 80) MEFs with cilia, after serum re-addition for 24 h. g Quantification of the percentage of ciliated MEFs out of total cells (~500 cells) at 24 h after serum re-addition. h Coronal sections of E16.5 cerebral cortex stained with antibodies against acetylated α-tubulin (red) and γ-tubulin (green). Control or Cep170 shRNA together with H2B-GFP constructs were injected into E14.5 ventricles. Right panels are enlargements of regions outlined by white dotted boxes in left panels. Scale bar: 5 μm (left panels); 1 μm (right panels). i Quantification of cilium length within a 1.153 × 105 μm2 field in h. j Quantification of cilium length in WT (n = 40) and CEP170 knockdown (n = 51) human NPCs. Error bars represent SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. represents no significant difference detected (Student’s t-test)
