Fig. 5
Brain leptin signaling has preserved anti-steatotic effects in obese animals. a Study timeline of HFD feeding combined with the chronic ICV leptin/vehicle infusion protocol. Note that we used a 3-time higher ICV leptin dose compared to previous experiments on regular chow (Fig. 1f). b Hepatic lipid content after 8 weeks of HFD feeding with ICV leptin/vehicle infusion during the last 4 weeks (n ≥ 5 per group). c Western blot analyses of liver tissue lysates (n ≥ 5 per group). d Quantification of the Western blot analyses in Fig. 5c. All data are mean ± SEM; **p < 0.01; ***p < 0.001; vs. vehicle by two-tailed Student’s t test; open circles: ICV vehicle; black squares: ICV leptin
