Fig. 3
Similar subcellular localization of WT1 with YAP. a Immunofluorescence staining for WT1 in epithelial cell sheets on flat substrata and NRA. Translocation of WT1 into nuclei was observed in marginal zones and FLPs of sheets expanding on NRA (brown boxes). Submarginal cells on NRA (red boxes) and on flat substrata showed YAP in the cytoplasm. The samples were fixed after 8 h to remove stencils. b Control and YAPKD cells and their nuclear fractions were analyzed using immunoblotting using YAP and WT1 antibodies. c Immunofluorescence staining for WT1 in cells and d immunoblotting of YAP and WT1 abundance in the nuclei in different density–epithelial cell sheets showing density-dependent translocation of WT1 into the nucleus
