Fig. 5
Cell migration-enhancing effect of YAP via Rac1. a Rac1 activity assay using cells cultured on flat surfaces and NRA (n = 3 biologically independent samples, * = statistical significance of the ratio of active Rac1 to Rac1 on flat surfaces vs. NRA. *P < 0.05). b Control, YAPKD, and YAPOE cells were immunoblotted to evaluate Rac1 activity via pull-down assay, expression of TRIO, and phosphorylation of PAK and Merlin. c Cell migration speed of individual cells in YAPKD (left) and YAPOE (right) cells in the marginal region of epithelial cell sheets on NRA in the presence of Rac1 inhibitor, NSC23766 and a TRIO inhibitor, ITX3 (each number of independently analyzed cells, n, is indicated, * = statistical significance of control vs. YAPKD and control vs. YAPOE cells with drugs, n.s = no significance, *P < 0.05, **P < 1 × 10−2, and ***P < 5 × 10−3). All error bars are S.E.M and statistical significance was determined by two-sided Student’s t-test
