Fig. 4

Proliferation of mesenchymal stem/progenitor cells is enhanced in miR-204/-211 dKO mice. a, b Representative flow cytometry profile (a) and quantification (b) of CD29⁺/CD105⁺/Sca-1⁺ cells in CD45⁻ synoviocytes freshly isolated from control and dKO mice. n = 3. c IHC results of proliferating cell nuclear antigen (PCNA) in control and dKO mice. Red arrowheads, PCNA-positive cells. Scale bars (left panels), 250 μm. Scale bars (right panels), 100 μm. d, e Representative flow cytometry profile (d) and quantification (e) of BrdU-labeled CD45⁻ cells, which were in vivo labeled and freshly isolated. n = 3. f Quantitative RT-PCR analysis of p16, p21, p27 and p57 expression in CD45⁻ BMSCs from control or dKO mice. n = 3. Unpaired Student’s t test. Data are shown as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001