Fig. 2 | Nature Communications

Fig. 2

From: Long-term memory is formed immediately without the need for protein synthesis-dependent consolidation in Drosophila

Fig. 2

cLTM requires dopaminergic neurons, but not the mushroom body. a Top: protocols. Temperature shifting is finished immediately before training to avoid inconsistency in temperature conditions. Bottom: blockade of dopaminergic neurons with TH-Gal4 and UAS-Shits during training abolishes context-dependent long-term memory (cLTM) formation (n = 8–10). b Both mutant (dDA1dumb2) and knockdown dDA1 in pan neurons (nSyb-Gal4;UAS-dDA1-RNAi) abolish cLTM formation. Selective overexpression of dDA1WT in mushroom body (MB) in dDA1dumb2 flies (dumb2;OK107-Gal4) does not rescue cLTM. Selective knockdown of dDA1 in the MB (OK107-Gal4;UAS-dDA1-RNAi) does not impair cLTM (n = 6–10). c, d Top: protocols. Temperature shifting is finished immediately before testing. Bottom: MB output is dispensable during cLTM retrieval (n = 6–14). Data are mean performance indices ± SEM; individual data points are displayed as dots; *P < 0.05 by ANOVA or t test

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