Fig. 6
From: Anatomy and function of the vertebral column lymphatic network in mice
Epidural and dural lymphatic drainage. a Schematic representation of the procedure used to test spinal cord drainage: LYVE1 Ab or OVA-A555 was injected in the thoraco-lumbar (Th-Lb) region of the spinal cord parenchyme. Fifteen or forty-five minutes later, mice were sacrificed. LYVE1 was detected by labeling with a secondary antibody, while OVA-A555 was directly identified by fluorescence detection. Schema is adapted from Fig. 2c in ref. 59. b–e LYVE1 Ab uptake by epidural and dural lymphatic circuits after 45 min. b LYVE1 Ab (purple) injection area (black dotted line) and epidural LVs recapture (white arrows). c–e Colocalization of LYVE1 with LVs around DRG, in contact with dura mater (white arrowheads). Asterisk: vertebral ventral body, SC: spinal cord. f OVA-A555 injection leads to labeling of ipsilateral mediastinal lymph node (MeLNs) after 15 min. g Schematic representation of the lymphatic drainage pattern in the thoracic vertebral column. Thoracic vertebrae (gray), dura mater and CSF (purple), LVs of epidural space (green), extravertebral LVs and lymph node (blue). Blue dots: accumulation of OVA-A555 tracer in LVs and lymph node. MeLN: mediastinal lymph node; PvLV: paravertebral lymphatic vessel. Scale bars: 200 µm (b–e); 1 mm (f)
