Fig. 4

TAF1 knockdown significantly delays leukemia development. a Kaplan–Meier plots show that TAF1 KD significantly extends the survival of recipient mice transplanted with AE9a+ luciferase+ cells. Sublethally irradiated C57BL/6J mice were injected with AE9a+ luciferase+ cells transduced with scrambled shRNA or mouse TAF1 shRNAs (n = 8 in each group). p value was determined using Log-rank (Mantel–Cox) test. b In vivo luciferase imaging indicates that knockdown of TAF1 remarkably impairs leukemia development (n = 8 in each group). Mice were injected with AE9a+ luciferase+ cells expressing wild-type level or reduced levels of TAF1. Twenty days after transplantation, IVIS imaging was performed. c The quantification of total luciferase signal in each mouse of each group as shown in (b). The luciferase signal in the TAF1 KD group was compared to the signal in the scrambled shRNA control group. d KD of TAF1 reduces the presence of GFP+ AE9a+ luciferase+ cells in the peripheral blood. Mice were injected with GFP+ AE9a+ luciferase+ cells infected with scrambled shRNA or TAF1 shRNAs. The percentage of GFP+ AE9a+ luciferase+ cells in peripheral blood of each mouse was measured 3 weeks after transplantation. The percentage of GFP+ AE9a+ in the peripheral blood of the TAF1 KD group was compared with the percentage of GFP+ cells in the scrambled shRNA group. P values were determined by Student's t-test. e Survival curves of mice injected with secondary spleen leukemia cells transduced with scrambled shRNA or TAF1 directed shRNAs. n = 8 mice in each group. P value was determined using Log-rank (Mantel–Cox) test. f The percentage of GFP+ AE9a+ cells in the peripheral blood of each mouse after receiving secondary spleen leukemia cells infected with scrambled shRNA or TAF1-directed shRNAs. Peripheral blood was collected 48 days after transplantation. The percentage of GFP+ AE9a+ cells in peripheral blood in the TAF1 KD group was compared with the percentage for the scrambled shRNA group. P values were determined by Student's t-test. *p < 0.05, **p < 0.01, ****p < 0.0001