Fig. 5

TAF1 associates with acetylated K43 on AE through its bromodomains. a TAF1 physically interacts with AE in Kasumi-1 cells. Co-immunoprecipitation was performed using anti-TAF1 antibody or normal mouse IgG. b Co-immunoprecipitation of TAF1 with AE using anti-ETO antibody or normal goat IgG. c Mutation of lysine-43 to arginine in AE reduces the interaction of AE with TAF1. 293T cells were transfected with p300, TAF1 and AE or AE mutants. Co-immunoprecipitation was performed using an anti-TAF1 antibody. d The deletion of the TAF1 bromodomain regions impairs its binding to AE. 293T cells were transfected with p300, AE, and TAF1 wild type or bromodomain deletion (ΔBr) plasmids. Co-immunoprecipitation was performed using an anti-ETO antibody