Fig. 6

TAF1 knockdown affects the expression of AE target genes. a, b The influence of AE knockdown (a) or TAF1 knockdown (b) on the expression of AE target genes in Kasumi-1 cells. Kasumi-1 cells were transduced with scrambled shRNA or AE shRNAs (a) or TAF1 shRNAs (b) for 5 days. mRNA levels of individual genes were standardized by the level of 18S rRNA. c Depletion of TAF1 impairs the expression of AE target genes in AE9a+ cells. AE9a+ cells were transduced with scrambled shRNA or TAF1 shRNAs for 5 days and the mRNA levels of individual genes were measured and standardized by mouse 18S rRNA levels. d The protein levels of AE target genes in Kasumi-1 cells and K562 cells infected with scrambled shRNA or TAF1 shRNAs. e, f Venn diagrams of the numbers of genes downregulated (e) or upregulated (f) (q < 0.05) in Kasumi-1 cells after TAF1 KD (shTAF1) or AE KD (shAE). Scrambled shRNA-infected cells were used as controls. Kyoto Encyclopedia of Genes and Genomes (KEGG 2016) gene set analysis was performed on genes downregulated (e) or upregulated by both TAF1 KD and AE KD. a–c Experiments were repeated independently three times and all bars represent the mean ± SD