Fig. 2

JIL-1 is unstable in absence of JASPer in the JASPer^cw2/cw2 mutant and in cell lines. a Gene model for P-element excision in the JASPer locus to generate JASPer^cw2 allele. The mRNA isoforms RA and RB are shown below. The excised genomic portion is marked in white and EP denotes the position of the excised P-element in EP-element line GS3268. b Western blot analysis of salivary gland extracts from L3 larvae of homozygous JASPer^cw2/cw2 and JIL-1^z2/z2 mutants and wild type larvae as control. Western blots using α-JIL-1, α-JASPer, α-H3S10ph, and α-H3K9me2 antibodies are shown, western blot with α-tubulin antibody was used as loading control. c Immunofluorescence microscopy of polytene chromosome squashes from L3 larvae of homozygous JASPer^cw2/cw2 and wild type larvae as control. From left to right, staining for JASPer, JIL-1, merged images and DNA are shown. The X chromosome is marked by arrow heads. Source data are provided as a Source Data file. d Immunofluorescence microscopy of polytene chromosome spreads from L3 larvae of homozygous JASPer^cw2/cw2 and wild type larvae as control. From left to right, staining for H3S10ph, H3K9me2, merged images and DNA are shown. The X chromosome is marked by arrow head and the chromocenter is labeled with “CC”. e Table summarizing viability of male and female JASPer^cw2/cw2 mutant flies. f Representative western blot analysis using α-JASPer and α-JIL-1 antibodies on whole cell extracts from S2 cells (left panel) and Kc cells (right panel) after jasper or jil-1 RNAi treatment, as used for RNA-seq experiments. A cross-reacting band is marked by asterisk. Source data are provided as a Source Data file. g Bar chart showing mean log₂ fold-change of normalized mean RNA-seq counts for JIL-1 and JASPer RNAi. Left panel, JIL-1 mRNA mean log₂ fold-change upon jasper RNAi (S2 n = 4 and Kc n = 4). Right panel, JASPer mRNA mean log₂ fold-change upon jil-1 RNAi (S2 n = 5 and Kc n = 4). Error bars represent standard error of the mean.