Fig. 1: Characterization of human desmin-positive, PAX7- negative cell populations.
From: Human muscle-derived CLEC14A-positive cells regenerate muscle independent of PAX7
a Experimental design. Cell colonies grow out of human muscle fiber fragments (HMFF) within 3 weeks after hypothermic treatment. b Absence of PAX7 transcripts in PAX7null cells. The PAX7 c.86-1G > A mutation in PAX7null cells leads to deletion of exon 2 and a premature stop codon in exon 3. The PCR primers shown here recognize exons 4 and 5. PAX7neg-B cells are derived from donors with intact Pax7 gene and also do not express PAX7. c Expression of markers of myogenic and interstitial cells assessed by qPCR in PAX7null, PAX7neg, and PAX7pos cell colonies. All data were normalized to two reference genes and relativized to the mean of PAX7pos cells. The dotted line at twofold and 0.5-fold represents our threshold for differential expression. d Myogenic cells derived from HMFFs were stained for PAX7, desmin, and MYF5. PAX7-negative populations (PAX7null, PAX7neg) show no specific signal for PAX7 in immunofluorescence stainings. Cell lines are described in Supplementary Table 2. Scale bars: 20 µm.
