Fig. 3: Define the in vivo molecular function of a trait-associated lncRNA.
From: In vivo functional analysis of non-conserved human lncRNAs associated with cardiometabolic traits
a Left: Relative expression levels of LINC01018 in human tissues of skeletal muscle (set as 1), liver, heart, pancreas, placenta, brain, kidney and lung analyzed by quantitative real-time PCR using human cDNA tissue panel from Clontech. Right: in vitro translation analysis using RNAs of YFP and LINC01018. b Hepatic expression of LINC01018 in response to fasting in humanized mice (n = 4 for each group) generated with human primary hepatocytes from two different donors. c Left: an illustration depicting the strategy to identify potential LINC01018 target genes. Right: in top pathways overrepresented by LINC01018 correlated protein-coding genes, the potential LINC01018 target genes are highlighted in red, which are also regulated by fasting in humanized mice together with LINC01018. d Expression levels of LINC01018 and its potential target genes in the livers of control and LINC01018 knockdown (KD) humanized mice received shRNA adenoviruses for LacZ (LacZ sh, n = 8) and LINC01018 (LINC01018 sh1, n = 8) respectively. e Gene expression in the livers of control (LacZ sh, n = 6) and LINC01018 KD (LINC01018 sh1, n = 6) humanized mice using human primary hepatocytes from a second donor after a 24 h food withdrawal. Data in (b, d, e) represent mean ± SEM, *p < 0.05, two-tailed unpaired Student’s t-test. Source data of (a, b, d, e) are provided in the Source Data file.
