Fig. 4: HuR mediates the regulatory effects of LINC01018.
From: In vivo functional analysis of non-conserved human lncRNAs associated with cardiometabolic traits
a Immunoblotting analysis of proteins in extract of humanized liver tissues that bind to biotinylated LINC01018 or a control using an anti-HuR antibody. b Left, anti-HuR immunoblotting analysis of proteins in immunoprecipitates of humanized liver tissues using an anti-HuR antibody. Right, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and LINC01018 RNA levels in immunoprecipitates of humanized liver tissues using an anti-HuR antibody. c. Expression levels of human HuR and LINC01018 target genes in the livers of control (LacZ sh, n = 5) and HuR KD (HuR sh, n = 6) humanized mice after a 24 h food withdrawal. d Gene expression in livers of humanized mice receiving both control (LacZ shRNA) and HuR KD, or LINC01018 KD and HuR KD adenoviruses (n = 7 for each group). e Gene expression in livers of wild-type mice receiving control (LacZ shRNA) or mouse HuR KD adenoviruses (n = 6 for each group). f Gene expression in livers of wild-type mice receiving control or LINC01018 overexpression (OE) adenoviruses (n = 6 for each group). g Relative RNA levels in anti-HuR immunoprecipitates using liver tissue lysates of wild-type mice receiving control or LINC01018 OE adenoviruses (n = 3 for each group). Data in (c–g) represent mean ± SEM, *p < 0.05, two-tailed unpaired Student’s t-test. h Relative expression of human CYP4A11 in 293 A cells treated with actinomycin D for 0, 2 and 4 h. Data represent mean ± SEM of three independent experiments, *p < 0.05 for “LINC01018 + HuR” compared with “Con + Con”, #p < 0.05 for “Con + HuR” compared with “Con + Con”, two-tailed unpaired Student’s t-test. Source data of (a–h) are provided in the Source Data file.
