Fig. 1: High TRIB3 expression is negatively associated with overall survival and metastasis-free survival in breast cancer. | Nature Communications

Fig. 1: High TRIB3 expression is negatively associated with overall survival and metastasis-free survival in breast cancer.

From: TRIB3 supports breast cancer stemness by suppressing FOXO1 degradation and enhancing SOX2 transcription

Fig. 1

a Graph derived from published data available in the Oncomine database. The box charts depict the relative expression of TRIB3 in invasive ductal (top) and invasive lobular (bottom) breast cancer patients. Centre line = 50th percentiles; bounds of box = 25th and 75th percentiles; bars = 10th and 90th percentiles; whiskers = min and max values. b Formalin-fixed, paraffin-embedded tissue microarray sections of normal and breast cancer tissues were stained with an anti-TRIB3 antibody. Tissue-bound TRIB3 is shown in brown (left). The dot plots show the relative expression of TRIB3 calculated by the intensity of the brown color in tissue array immunohistological images (right). c Immunoblots of protein lysates from HMLE, HMLER, and BCCs, as indicated at the top of the left panel. The relative TRIB3 expression quantification from three independent studies is shown (right). d, e Graph derived from TCGA (d) or published data (e) available in the PubMed GEO database (GSE2603, GSE5327, GSE2034, and GSE12276). For each analysis, 1117 patients (d) or 582 patients (e) were segregated into tertiles, with the group designated TRIB3H representing one-third of the patients who had tumors with the highest levels of TRIB3 mRNA and the group designated TRIB3L representing one-third of patients who had cancers with the lowest levels of TRIB3 mRNA. One-third of patients who had tumors with intermediate TRIB3 mRNA expression were designated as TRIB3M. Overall survival (d) and metastasis-free survival (e) were determined by Kaplan–Meier analyses, and significant differences were determined by the log-rank test. The number of patients in each category, the total metastatic events, and the corresponding P-values (χ2-test) are shown in the embedded tables. f–i Flow cytometry of CD45−CD31−TER119−(Lin−) MECs from the mammary glands of 6-week-old MMTV-PyMT female mice (f) and 6-month-old MMTV-ErbB2 female mice (h). Relative Trib3 mRNA expression and TRIB3 protein expression are indicated in g and i. Data are presented as the mean ± SEM; P > 0.05 was considered not significant (NS); *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the HMLE group in c and the normal gland group in g and i. Source data are provided as a Source Data file.

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